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We hypothesized that the muscle protein anabolic resistance to amino acids occurs in older adults and that RET could overcome such anabolic resistance by enhancing mTORC1 signaling and MPSresponse in this population. As a first step, we measured the MPS response and basal anabolic sensitivity of human muscle from older adults (64–79 y) with and without RET, and compared the responses between RET and both saline and amino acid control conditions with no RET (Figure 1). We found that muscle protein synthesis was significantly greater after RET of younger adults, although not older adults (P = 0, amino anabolic.01), amino anabolic. The magnitude of the response after RET by younger adults was approximately 2.3-fold greater than after amino acid control (P < 0.05), but was only 2.0-fold greater (P < 0.05) in older adults (P value in between; Figure 1). After RET, the basal anabolic rate was elevated with 2, cheap steroids canada.6-fold greater protein synthesis (in the old vs young: 0, cheap steroids canada.18 vs 0, cheap steroids canada.13, respectively; P < 0, cheap steroids canada.05) after protein loading (2–4 g in young adults as compared with 3–4 g in older adults; P = 0, cheap steroids canada.02) but not in young adults with RET (P = 0, cheap steroids canada.58), cheap steroids canada. Moreover, the mTORC1 signaling (in the old vs young: 1, cheap steroids canada.36 vs 0, cheap steroids canada.99 [P < 0, cheap steroids canada.01], respectively) was only slightly enhanced after RET (1, cheap steroids canada.20) in the young but 1, cheap steroids canada.37-fold greater in old adults (Figure 1), cheap steroids canada. FIGURE 1 View largeDownload slide Muscle protein synthesis (A) and anabolic sensitivity (B) of young (left) [−0.2 μmol · kg−1 · min−1 (old vs young)] and older adults (right) who received RET after ingestion of 20 g protein (20 g of casein) over 3 d (12 h) in fasted state. Young adults who received the first dose of RET consumed the protein immediately after waking, anavar geneza. All samples analyzed were obtained after 1 h of recovery, anabolic amino. The values of the young adults were normalized to the values of the corresponding values from the older subjects (P < 0.05, two-tailed t test). FIGURE 1 View largeDownload slide Muscle protein synthesis (A) and anabolic sensitivity (B) of young (left) [−0.2 μmol · kg−1 · min−1 (old vs young)] and older adults (right) who received RET after ingestion of 20 g protein (20 g of casein) over 3 d (12 h) in fasted state.
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